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Waltham, MA, USA) staining as adapted and modified from published protocols.[12] Briefly, cells were washed twice with PBS, fixed with 10% formalin for 30 min at room temperature, and stained with oil red O working solution (~0.2% oil red O in 99% isopropanol) for 30 min

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Pick an Injection Site: Use the abdomen (at least 2 inches from the belly button), thigh, or upper arm